Teko e potlakileng ea TOXO IgG/IgM

Mokhoa oa ho hlahloba
Ho na le mekhoa e meraro ea mantlha ea tlhahlobo ea toxoplasmosis: tlhahlobo ea pathogenic, tlhahlobo ea immunological le tlhahlobo ea limolek'hule.Tlhahlobo ea pathogenic haholo-holo e kenyelletsa tlhahlobo ea histological, ente ea liphoofolo le ho itšehla thajana, le setso sa lisele.Mekhoa e tloaelehileng ea tlhahlobo ea serological e kenyelletsa tlhahlobo ea dae, tlhahlobo ea hemagglutination e sa tobang, tlhahlobo e sa tobang ea immunofluorescence antibody le enzyme e amanang le immunosorbent assay.Tlhahlobo ea limolek'hule e kenyelletsa theknoloji ea PCR le theknoloji ea nucleic acid hybridization.
Tlhahlobo ea 'mele ea moimana ea baimana e kenyelletsa tlhahlobo e bitsoang TORCH.TORCH ke motsoako oa tlhaku ea pele ea lebitso la Senyesemane la likokoana-hloko tse 'maloa.Tlhaku T e emetse Toxoplasma gondii.(Mangolo a mang a emela syphilis, kokoana-hloko ea rubella, cytomegalovirus le kokoana-hloko ea herpes simplex ka ho latellana.)
Hlahloba molao-motheo
Tlhahlobo ea pathogen
1. Tlhahlobo e tobileng ea microscopic ea mali a mokuli, moko oa masapo kapa cerebrospinal fluid, pleural le ascites, sputum, bronchoalveolar lavage fluid, motlae oa metsi, amniotic fluid, joalo-joalo bakeng sa smears, kapa lymph nodes, mesifa, sebete, placenta le lisele tse ling tse phelang. likarolo, bakeng sa tlhahlobo ea Reich kapa Ji staining microscopic e ka fumana trophozoites kapa cysts, empa sekhahla se setle ha se holimo.E ka boela ea sebelisoa bakeng sa immunofluorescence ka ho toba ho lemoha Toxoplasma gondii ka lisele.
2. Ente ea liphoofolo kapa setso sa 'mele Nka mokelikeli oa 'mele kapa ho emisoa ha lisele ho ea hlahlojoa le ho e kenya ka mpeng ea litoeba.Tšoaetso e ka hlaha 'me likokoana-hloko li ka fumanoa.Ha moloko oa pele oa ente e le mpe, e lokela ho fetisoa ka bofofu ka makhetlo a mararo.Kapa bakeng sa setso sa lisele (liphio tsa monkey kapa lisele tsa liphio tsa kolobe) ho arola le ho khetholla Toxoplasma gondii.
3. Thekenoloji ea hybridization ea DNA Litsebi tsa malapeng li ile tsa sebelisa li-probes tse ngotsoeng ka 32P tse nang le tatellano e khethehileng ea DNA ea Toxoplasma gondii ka lekhetlo la pele ho tsamaisa motsoako oa limolek'hule ka lisele kapa lisele tsa DNA maling a peripheral ea bakuli, 'me li bontšitse hore lihlopha tse khethehileng tsa hybridization kapa matheba e ne e le liketso tse ntle.Bobeli bo ikhethang le kutloisiso li ne li phahame.Ho feta moo, polymerase chain reaction (PCR) e boetse e thehiloe Chaena ho hlahloba lefu lena, 'me ha e bapisoa le probe hybridization, ente ea liphoofolo le mekhoa ea tlhahlobo ea immunology, e bontša hore e totobetse haholo, e hlokolosi ebile e potlakile.
Tlhahlobo ea Immunological
1. Li-antigen tse sebelisetsoang ho lemoha li-antigen haholo-holo li kenyelletsa tachyzoite soluble antigen (cytoplasmic antigen) le membrane antigen.Antibody ea pele e ile ea hlaha pejana (e fumanoe ka tlhahlobo ea litšila le tlhahlobo e sa tobang ea immunofluorescence), ha ea morao-rao e hlaha hamorao (e fumanoe ka tlhahlobo e sa tobang ea hemagglutination, joalo-joalo).Ka nako e ts'oanang, mekhoa e mengata ea ho lemoha e ka bapala karolo e tlatsetsang le ho ntlafatsa sekhahla sa ho lemoha.Kaha Toxoplasma gondii e ka ba teng liseleng tsa motho ka nako e telele, ho thata ho khetholla tšoaetso ea hona joale kapa tšoaetso ea nakong e fetileng ka ho lemoha li-antibodies.E ka ahloloa ho latela titer ea antibody le liphetoho tsa eona tse matla.
2. Antigen ea ho lemoha e sebelisetsoa ho lemoha likokoana-hloko (tachyzoites kapa cysts) ka lisele tse amohelang, metabolites kapa lihlahisoa tsa lysis (li-antigen tse potolohang) ka serum le metsi a 'mele ka mekhoa ea immunology.Ke mokhoa o tšepahalang oa tlhahlobo ea pele le tlhahlobo e hlakileng.Litsebi tsa malapeng le linaheng tse ling li thehile McAb ELISA le sandwich ELISA pakeng tsa McAb le multiantibody ho lemoha ho potoloha ha antigen ka serum ea bakuli ba mahlomoleng, ka kutloisiso ea 0.4 μ G/ml ea antigen.