Teko ea Antigen ea Rota virus

Letšollo ke e 'ngoe ea lisosa tsa mantlha tsa ho kula le ho shoa ha bana lefatšeng ka bophara, le bakang mafu a limilione tse 2,5 selemo le selemo.Tšoaetso ea Rotavirus ke sesosa se ka sehloohong sa letšollo le matla ho masea le bana ba ka tlaase ho lilemo tse hlano, e leng karolo ea 40-60% ea lefu la ho ruruha ha mpa le ho baka mafu a hakantsoeng a 500,000 a bana selemo le selemo.Ha a le lilemo li hlano, hoo e ka bang ngoana e mong le e mong lefatšeng o se a tšoaelitsoe ke rotavirus bonyane hang.Ka tšoaetso e latelang, karabo e pharaletseng, ea heterotypic antibody e hlahisoa;ka hona, ha se hangata batho ba baholo ba amehang.Ho fihlela joale lihlopha tse supileng tsa rotavirus (lihlopha AG) li arotsoe 'me li khetholloa.Sehlopha sa A rotavirus, e leng rotavirus e tloaelehileng ka ho fetisisa, e baka ho feta 90% ea tšoaetso eohle ea Rotavirus bathong.Rotavirus e fetisoa haholo-holo ka tsela ea molomo oa mantle, ka kotloloho ho tloha ho motho ho ea ho motho.Li-titer tsa kokoana-hloko ka mantle li fihla holimo nakoana ka mor'a ho qala ho kula, ebe lia fokotseha.Nako ea ho incubating ea tšoaetso ea rotavirus hangata ke letsatsi le le leng ho isa ho a mararo 'me e lateloa ke lefu la mala ka nako e tloaelehileng ea matsatsi a mararo ho isa ho a supileng.Matšoao a lefu lena a fapana ho tloha ho letšollo le fokolang, le nang le metsi ho ea ho letšollo le matla le feberu le ho hlatsa.Tlhahlobo ea tšoaetso ea rotavirus e ka etsoa ka mor'a ho hlahlojoa ha gastroenteritis e le sesosa sa letšollo le matla ho bana.Haufinyane tjena, tlhahlobo e khethehileng ea ts'oaetso e nang le rotavirus e fumaneha ka ho fumanoa ha antigen ea kokoana-hloko setulong ka mekhoa ea immunoassay e kang latex agglutination assay, EIA, le lateral flow chromatographic immunoassay.Rotavirus Ag Rapid Test ke lateral flow chromatographic immunoassay e sebelisang para ea li-antibodies tse khethehileng ho lemoha ka boleng antigen ea rotavirus mokhoeng oa mantle.Teko e ka etsoa ntle le lisebelisoa tse boima tsa laboratori, 'me liphetho li fumaneha nakong ea metsotso e 15.

Rotavirus Ag Rapid Test ke lateral flow chromatographic immunoassay.

Lethathamo la liteko le na le:

1) letlapa le nang le 'mala oa burgundy le nang le anti-rotavirus ea monoclonal e kopantsoeng le khauta ea colloidal (li-anti-rotavirus conjugates) le antibody e laolang e kopantsoeng le khauta ea colloidal.

2) lesela la membrane la nitrocellulose le nang le mohala oa teko (T line) le mohala oa taolo (C line).

Mohala oa T o kentsoe pele ho anti-rotavirus e 'ngoe ea monoclonal,' me mohala oa C o kentsoe pele ka lesole la 'mele la taolo.