Leqephe le sa Khaoletsoang la Qrf Antibody

Qrf Antibody Test

Mofuta: Leqephe le sa Khaohang

Lebitso: Bio-mapper

Lethathamo la lintlha: REA0911

Mohlala: WB/S/P

Setsi sa tlhahlobo ea kokoana-hloko ea kokoana-hloko ea kokoana-hloko ea linku e sebelisoa ho lemoha li-anti-pox tsa linku ka har'a serum ea linku, e ka sebelisoang ho lekola phello ea 'mele ea ho itšireletsa mafung ea ente ea pox ea linku kapa ho thusa ho lemoha lefu lena.Molao-motheo ke hore sesebelisoa sa ho lemoha likokoana-hloko tsa kokoana-hloko ea linku se entsoe ka poleiti ea antigen ea linku e koahetsoeng esale pele, matšoao a enzyme le li-reagents tse ling tse tšehetsang, 'me molao-motheo oa enzyme-linked immunoassay (ELISA) o sebelisoa ho lemoha pox ea linku. antibody ka har'a sampole ea serum ea linku.

Re romelle lengolo-tsoibila

Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Tlhaloso e qaqileng

Ho sibolloa ha kokoana-hloko ea kokoana-hloko ea kokoana-hloko ea linku ho entsoe ka microplate e koahetsoeng esale pele ka li-protein tsa nyutlelie ea linku, li-marker tsa enzyme le li-reagents tse ling tse tšehetsang, 'me molao-motheo oa enzyme-linked immunoassay (ELISA) o sebelisoa ho lemoha lets'oao la linku ka har'a mohlala oa serum ea linku.Nakong ea teko, serum ea taolo le sampole e tla hlahlojoa li eketsoa poleiting ea microplate, 'me haeba sampole e na le antibody ea pox ka mor'a ho kenngoa, e tla tlamelloa ho antigen ka poleiti ea microplate, le likarolo tse ling tse sa tlangoa. e tla tlosoa ka mor'a ho hlatsoa;Ebe u eketsa letšoao la enzyme ho tlama ka ho khetheha ho motsoako oa antigen-antibody holim'a poleiti ea microplate;Lits'oants'o tsa enzyme tse sa koaloang li ile tsa tlosoa ka ho hlatsuoa, 'me tharollo ea substrate ea TMB e ile ea eketsoa lilibeng,' me sehlahisoa se seputsoa se entsoe ke karabelo ea li-conjugate tsa microplate, 'me botebo ba' mala bo ne bo amana hantle le palo e itseng ea li-antibodies tse teng mohlala.Ka mor'a hore tharollo ea ho felisa e eketsoe ho felisa karabelo, sehlahisoa se ile sa fetoha mosehla;Boleng ba ho monya karabelong e 'ngoe le e' ngoe hantle bo khethoa ke sebali sa microplate se bolelele ba 450 nm ho fumana hore na sampole e na le li-antibodies tsa pox ea nku.